Error message

Deprecated function: The each() function is deprecated. This message will be suppressed on further calls in theme_biblio_custom() (line 367 of /home/phcogcommn/phcogcommn.org/sites/all/modules/biblio/includes/biblio_theme.inc).

Antibacterial Activities and Phytochemical Screening of Crude Extracts from Kenyan Macaranga Species Towards MDR Phenotypes Expressing Efflux Pumps

groupview

Abstract
Pharmacognosy Communications,2021,11,2,119-126.
Published:April 2021
Type:Original Article

Antibacterial Activities and Phytochemical Screening of Crude Extracts from Kenyan Macaranga Species Towards MDR Phenotypes Expressing Efflux Pumps

Ibrahim Hashim1,2, Leonidah Kerubo Omosa1,*, Vaderament-Alexe Nchiozem-Ngnitedem1, John Mmari Onyari1, Shital Mahindra Maru3, Michel-Gael Fofack Guefack4, Armelle Tsafack Mbaveng4, Victor Kuete4

1Department of Chemistry, University of Nairobi, Nairobi, KENYA.

2Department of Chemistry, Federal University Lafia, Nasarawa State, NIGERIA.

3Department of Pharmaceutics and Pharmacy Practice, University of Nairobi, Nairobi, KENYA.

4Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, CAMEROON.

Abstract:

Introduction: Macaranga species are traditionally used for the treatment and management of coughing, fungal infection, and wounds. In this study, the phytochemical screening and antibacterial activities of nine crude extracts from Macaranga conglomerata, Macaranga kilimandscharica and Macaranga capensis were determined against 13 bacterial strains expressing multi-drug resistance (MDR) phenotypes. Methods: Phytochemical screening of the extracts were carried out according to the standard methods, while the iodonitrotetrazolium chloride (INT) colorimetric assay was used to determine the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of the plants extracts. Results: Flavonoids, terpenoids, saponins and coumarins were the major secondary metabolites found in all the plant extracts. The results of antibacterial studies revealed that all the plant extracts displayed good activities with MIC values ranging from 4 – 128 μg/mL against the tested micro-organisms. Most of the extracts exhibited a bactericidal effect against E. coli, E. aerogenes, K. pneumoniae, P. stuartii, P. aeruginosa, and S. aureus with MBC/MIC ratio ≤ 4. In the presence of efflux pump inhibitor (PaβN), the inhibition potency of all the crude extracts against the tested bacterial strains were substantially enhanced. It is worth noting that the activities of MKL, MCL, and MCR towards P. stuartii (NEA16), E. aerogenes (ATCC13048), and K. pneumoniae (KP55), respectively were improved by more than 8-fold in the presence of PAβN. Conclusion: The findings of this study indicated the possibility of using all the tested plant extracts as a source of therapeutic agents in the fight against multi-drug resistant bacteria.

Founded:  In 2004, as the PHCOG.NET – a non-profit private organization dedicated to Natural Products Research leading to develop promising drugs. Our main mission is to make information on herbal drug research readily available in different formats to suit the individual needs.

Pharmacognosy Communications [Phcog Commn.] is a new quarterly journal published by Phcog.Net. It is a peer reviewed journal aiming to publish high quality original research articles, methods, techniques and evaluation reports, critical reviews, short communications, commentaries and editorials of all aspects of medicinal plant research. The journal is aimed at a broad readership, publishing articles on all aspects of pharmacognosy, and related fields. The journal aims to increase understanding of pharmacognosy as well as to direct and foster further research through the dissemination of scientific information by the  publication of manuscripts. The submission of original contributions in all areas of pharmacognosy are welcome.

The journal aims to cater the latest outstanding developments in the field of pharmacognosy and natural products and drug design covering but not limited to the following topics:

  • Pharmacognosy and pharmacognistic investigations
  • Research based ethnopharmacological evaluations
  • Biological evaluation of crude extracts, essential oils and pure isolates
  • Natural product discovery and evaluation
  • Mechanistic studies
  • Method and technique development and evaluation
  • Isolation, identification and structural elucidation of natural products
  • Synthesis and transformation studies

Distinctions:  The most widely read, cited, and known Pharmacognosy Communications and website is well browsed with all the articles published. More than 20,000 readers in nearly every country in the world each month

Aim and Scope | Editorial Board | Indexed & Abstracted | Instruction to Authors | Manuscript Submission & Charges

Subjects Covered : Natural Products, Pharmacognosy, Phytochemistry, Marine Pharmacognosy and Zoo Pharmacognosy
ISSN :2249-0159 (Print) ; 2249-0167 (Online) Frequency : Quarterly Rapid at a time publication (4 issues/year)

Indexed and Abstracted in : Chemical Abstracts, Excerpta Medica / EMBASE, Google Scholar, CABI Full Text, Ulrich’s International Periodical Directory, ProQuest, Journalseek & Genamics, PhcogBase, EBSCOHost, Academic Search Complete, Open J-Gate, SciACCESS.
Rapid publication: Average time from submission to first decision is 30 days and from acceptance to In Press online  publication is 45 days.
Open Access Journal: Phcog Commn. is an open access journal, which allows authors to fund their article to be open access from publication.